Image Sample Data

Transthyretin (55 kDa) resolved to 4.6 Å at 100 keV acquired with an Alpine™ camera on a Talos F200C (equipped with an SFEG) using CDS at ~5.2 e^-/pixel/s. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

Metro captures 4D STEM data with sharper detail and less background noise; it can collect a 128 x 128 (scan) x 512 x 512 (pattern) dataset in half a minute. This color image is a map of the brightest spot orientation and intensity from such a dataset. The material is boron nitride. The color hue in the map specifies the direction from center of the strongest spot in each diffraction pattern, while the brightness corresponds to the intensity of the peak. 

OneView can detect many faint diffraction spots, but the counted data from Metro provides sharper detail and less background noise, allowing you to more accurately characterize crystals and defect structures even at very low electron doses. Here faint spots from a Zeolite diffraction pattern are compared. The TEM settings were not altered when the cameras were switched. 

Low dose imaging of a beam-sensitive Zeolite sample. Detail is observed out to the Nyquist frequency of the camera, even at a dose rate of 0.74 e^-/Å_²/s and a total dose of just 25 e^-/Å_², allowing a large field of view to be imaged. This is made possible by electron counting. 

When combined with Latitude software, Alpine™ delivers high-quality data at the highest imaging throughputs from 100 – 200 keV.

Aldolase (100 kDa) resolved to 3.07 Å at 100 keV acquired with an Alpine™ camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

Apoferritin (550 kDa) resolved to 2.7 Å at 100 keV acquired with an Alpine™ camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

A 4D STEM diffraction data cube collected using the Stela camera and STEMx system. Specimen: Au nanoparticles. Scan area: 176 x 218 pixels, Diffraction image size: 256 x 256 pixels. Dwell time: 5 ms. Color map showing grain orientations classified based on the angular position of the maximum diffraction peak per probe position using a DigitalMicrograph script.

Solved with the K3 Direct Detection Camera; bioRxiv - June 17, 2020

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