Phosphorus-containing macromolecular assemblies in the cell nuclei of drosophila larvae
Data courtesy of Dr. Maria Aronova and Dr. Richard Leapman, National Institutes of Health, Bethesda, Maryland.
Image of phosphorus-containing macromolecular assemblies in the cell nuclei of drosophila larvae; dual-axis EFTEM tilt series acquired with a Tridiem energy filter from -66˚ to 66˚; for each axis phosphorus L-23 series (20 eV slit, pre-edge and post-edge) were collected; a jump-ratio series was calculated and the data were aligned and reconstructed using IMOD (University of Boulder, Colorado); finally, the reconstruction was rendered in the 3D visualization software; the inset highlights a sub-volume extracted using the 3D visualization ROI tool.
Interplanetary dust particle
Data courtesy of Dr. Ilke Arslan of Sandia National Laboratories, Livermore, California and Dr. John P. Bradley of Lawrence Livermore National Laboratory, Livermore, California.
Image of interplanetary dust particle; STEM tilt series acquired with a 806 HAADF detector from -61˚ to 68˚; aligned, reconstructed and visualized using 3D reconstruction and 3D visualization software; the iso-surfaces highlight two different intensity levels in the reconstruction.
First 3.2 Å β-galactosidase structure solved by cryo-EM
Bartesaghi, A.; Matthies, D.; Banerjee, S.; Merk, A.; Subramaniam, S.
Structure of Escherichia coli β-galactosidase (∼465 kDa) at 3.2 Å resolution solved using the GIF Quantum LS energy filter that includes a K2 Summit camera. The first atomic model derived using single-particle cryo-EM analysis closely matches the 1.7 Å crystal structure with a global rmsd of ∼0.66 Å. Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):11709-14.
Nano-crystals
Image courtesy of the University of California - Berkeley, Dr. Kent McDonald.
Nano-crystals recorded with UltraScan® 1000XP CCD camera on a 120 kV TEM.
Myelinated nerve
Image courtesy of Kenneth L. Tiekotter, University of Portland.
Image of myelinated nerve recorded with Orius® SC1000W (35 mm mount) CCD camera.
Methods
recorded at 85kx at 80 kV
Lingual nerve
Methods
recorded at 13500x, 120 kV
Kidney
Methods
recorded at 200x, 120kV
Dark field image from the encircled reflections in the SAED revealing nanoscale γ´´ and eta-Ni3Ti plate-shaped precipitates
Samples and images courtesy of Vijay K. Vasudevan, University of Cincinnati.
Dark field image from the encircled reflections in the SAED revealing nanoscale γ´´ and eta-Ni3Ti plate-shaped precipitates. The γ´´ has a DO22 structure, whereas the eta has a hexagonal structure. Inset: [011] SAED pattern from IN718 Ni-base superalloy showing reflections from the γ matrix, γ´´ precipitates and eta-Ni3Ti plate-shaped precipitates.
CBED pattern from LaCoO3
CBED pattern from LaCoO3 recorded with Orius SC200D CCD camera.
Methods
camera length of 135 mm
exposure time of 1 s (average of 30 frames)
spot size #9 at 200 kV
image is displayed on logarithm scale
SAED pattern from LaCoO3 (Image 2)
SAED pattern from LaCoO3 recorded in reverse contrast with Orius SC200D CCD camera.
Methods
camera length of 600 mm
exposure time of 10 s
spot size #4 at 200 kV
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