High-resolution image of C. elegans stomach sample

High-resolution image of C. elegans stomach sample
Captured with the Rio 16 camera; 200 kV; TEM indicated magnification: 6.3kx; image size: 4k x 4k; exposure time: 1 s.

OnPoint detector minimizes charging and beam damage

OnPoint detector minimizes charging and beam damage
Image courtesy of T. Deerinck NCMIR/UCSD.

Image shows that the OnPoint BSE detector can resolve features at 1.1 kV (right) that were previously distorted (e.g., black spots) by charging or beam damage at 2.2 kV (left). Sample: Mouse cerebellum. 

OnPoint increases 3View imaging speed four-fold

OnPoint increases 3View imaging speed four-fold

Compares image quality from the 1st generation 3View® detector (top) to the OnPoint™ detector (bottom) at various dwell times. Results show the OnPoint detector delivers equal or better resolution images using shorter dwell times (0.5 vs. 2.0 µs). Sample: Brain, 3 kV, 15 kx, 0.5 µs dwell time.

Preserves synaptic vesicle under low kV conditions

Synaptic vesicles
Image courtesy of T. Deerinck NCMIR/UCSD.

Demonstrates how low kV conditions can preserve and discriminate fine features, such as synaptic vesicles. Sample: 2 kV, 0.2 nm pixels, 24k x 24k image, 1 µs dwell time.

Gatan Microscopy Suite Software

GMS 3 software screen shot

EELS made easy with the new Gatan Microscopy Suite® (GMS) 3 software.

  • Advanced model-based quantification and mapping
  • STEM SI acquisition modalities
  • Experiment oriented workflow for fast time to results

The Efficient Frontier of Resolution

The Efficient Frontier of Resolution

Comparison between the resolution and molecule size for published single-particle cryo-EM structures.

Clearly resolved myelin sheaths and collagen fibers

Clearly resolved myelin sheaths and collagen fibers

The zoomed in image (right) obtained using the OneView camera shows clearly-defined detail within the myelin sheathes and collagen fibers when compared to the original image (left).

Sample: skin; beam energy: 120 kV; image size: 4k x 4k; exposure: 1 s; number of frames: 25

Fine details revealed in skin cells

Fine details revealed in skin cells

The zoomed in image (right) obtained using the OneView camera shows clearly-defined detail and resolution when compared to the original image (left).

Sample: skin; beam energy: 120 kV; image size: 4k x 4k; exposure: 1 s; number of frames: 25

Clearly resolved collagen fibrils

Clearly resolved collagen fibrils

Collagen fibrils are clearly resolved in this 4k x 4k image obtained using the OneView camera. This definition is maintained when the original image is zoomed in on during post-processing.

Sample: collagen; beam energy: 120 kV; image size: 4k x 4k; exposure: 1 s; number of frames: 25

High resolution image of C. elegans

High resolution image of C. elegans

OneView camera captures high resolution images of biological specimens, such as C.elegans at 3840 x 2160 pixels.

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