Image Sample Data

Metro captures 4D STEM data with sharper detail and less background noise; it can collect a 128 x 128 (scan) x 512 x 512 (pattern) dataset in half a minute. This color image is a map of the brightest spot orientation and intensity from such a dataset. The material is boron nitride. The color hue in the map specifies the direction from center of the strongest spot in each diffraction pattern, while the brightness corresponds to the intensity of the peak. 

OneView can detect many faint diffraction spots, but the counted data from Metro provides sharper detail and less background noise, allowing you to more accurately characterize crystals and defect structures even at very low electron doses. Here faint spots from a Zeolite diffraction pattern are compared. The TEM settings were not altered when the cameras were switched. 

Low dose imaging of a beam-sensitive Zeolite sample. Detail is observed out to the Nyquist frequency of the camera, even at a dose rate of 0.74 e^-/Å_²/s and a total dose of just 25 e^-/Å_², allowing a large field of view to be imaged. This is made possible by electron counting. 

When combined with Latitude software, Alpine™ delivers high-quality data at the highest imaging throughputs from 100 – 200 keV.

Aldolase (100 kDa) resolved to 3.07 Å at 100 keV acquired with an Alpine™ camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

Apoferritin (550 kDa) resolved to 2.7 Å at 100 keV acquired with an Alpine™ camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.

StelaカメラとSTEMxシステムを用いて取得した4D STEMデータ。試料：金ナノ粒子、スキャン範囲：176 × 218 ピクセル、回折図形サイズ：256 × 256 ピクセル、ドウェルタイム：5 ms　DigitalMicrographスクリプトを用いてビーム位置毎の回折スポットの角度方向の最大強度の位置を基に分類し結晶方位を示したカラーマップ。

Solved with the K3 Direct Detection Camera; bioRxiv - June 17, 2020

Virus-neutralizing antibodies are one of the approaches to treat or prevent infection by the COVID-19 virus.

Science - May 15, 2020

Preliminary results show remdesivir reduces recovery time for patients who receive the drug. The structure, solved by cryo-EM using Gatan equipment, shows how remdesivir blocks virus replication.