K2 camera helps identify first ~700 kDa protein structure with D7 symmetry at 3.3 Å resolution using cryo-EM

Li, X.; Mooney, P.; Zheng, S.; Booth, C. R.; Braunfeld, M. B.; Gubbens, S.; Agard, D. A.; Cheng, Y.

First ~700 kDa protein structure with D7 symmetry identified at 3.3 Å resolution using cryo-EM

Using the K2 Summit^® camera, single-particle cryo-EM was able to match the x-ray crystallography result. (d) Portion of the cryo-EM density map showing clear side-chain densities. The docked atomic structure was refined to fit the density map by a molecular dynamic flexible fitting procedure. (e) The same portion of a 2Fo – Fc map of 3.4-Å crystal structure calculated using the atomic structure (PDB: 1PMA). Nature Methods 10, 584 – 590 (2013).