Cerebellum (video 2)

The 3View^® 2 system offers life science researchers the ability to obtain in situ 3D data at remarkably fine depth resolution by operating a high-precision ultramicrotome within a field emission gun scanning electron microscope (FEGSEM). Based on the groundbreaking serial block-face scanning electron microscopy (SBFSEM) work performed by MPI Heidelberg, the revolutionary 3View 2 system allows automated acquisition of 3D ultrastructure by sequentially imaging a freshly cut, resin-embedded block face. Unlike the 3D imaging techniques in light microscopy, the spatial resolution provided by 3View 2 system in the Z direction is comparable to its X-Y resolution and does not degrade with depth.