There are a number of options and technologies available for digital imaging in transmission electron microscopy (TEM) applications today. Traditionally, high energy electrons could not be directly exposed to a sensor without excessively damaging the detector. As a consequence, conventional TEM cameras first expose the incoming electron beam to a scintillating film that converts the electrons into light (photons). These photons are then transferred to the sensor, either through a series of optical lenses or a coupled fiber optic plate. Finally, the light is collected by a sensor where the image is created pixel-by-pixel based on the amount of light detected at each position in the sensor.
Conventional TEM image detection architecture
There are four basic steps in TEM imaging to address incoming electrons:
- Convert electrons to signal
- Transfer signal
- Detect signal with sensor
- Electronically transfer signal and read-out to form image
What is different with direct detection?
There are only two steps in TEM imaging with direct detection:
- Convert electrons to signal – not applicable
- Transfer signal – not applicable
- Detect signal with sensor
- Electronically transfer signal and read-out to form image
One key difference between conventional and direct detection is a custom CMOS sensor that utilizes the only radiation hard architecture that can tolerate direct exposure to high energy particles. To add, extremely high speed electronics for data transfer and processing enable low-dose counting and super-resolution capabilities. Combined, this allows frame rates (4k x 4k) of 400 frames per second (fps) to be processed in real-time to achieve optimal results.
Convert electrons to signal Transfer signal Detect signal with sensor Transfer signal and read-out image
Step 1) Convert electrons to signal
Gatan uses proprietary phosphor scintillators to optimize signal conversion that enhances detector sensitivity (SENS) and resolution. When you select a scintillator, it is appropriate to know the performance trade-offs between SENS and resolution.
- Sensitivity (signal): Ideal for dose-sensitive use cases where you need to generate more photons per incoming electron (e.g., cryo-tomography, beam sensitive materials)
- Resolution (spatial detail): Favorable for applications where you require more information to resolve details, but you can increase the dose (signal) without harming the sample (e.g., semiconductors and other less-sensitive materials)
Step 2) Transfer signal
Various coupling (lens- and fiber-coupled) mechanisms are available to optimize signal transfer and meet cost or performance targets for a given detector.
Lens-coupled: Lens optics transmit light to the sensor where it is converted into sensor electrons (signal)
- Pros: (Gatan) Uses real transmission scintillator; can be less expensive than higher performance fibers
- Cons: Light (information) loss with lensing, angular dependencies (<10% efficient); vignetting (light fall-off); image distortion for higher magnification
Fiber-coupled: Scintillator creates photons that subsequently create sensor electrons; fiber directly transmits light to sensor with high efficiency
- Pros: Most efficient transmission of light information to the sensor (1:1 coupling of scintillator:sensor (>50% efficient) with no image distortion); can trade-off SENS verses resolution (fiber configuration detail)
- Cons: Fiber optics are slightly higher cost; requires process optimization including cladding, sintering, bonding of fibers (Gatan proprietary)
Step 3) Detect signal with sensor
Sensor type (CCD vs. CMOS) offers significant trade-offs for TEM camera performance as there are fundamental differences in architecture.
- Charge-coupled device (CCD): Charge transfers between neighboring cells, and read-out (e.g., noise) is seen at final stage; binning minimizes the impact of read-out noise
- Complementary metal–oxide–semiconductor (CMOS): Charge immediately converts to voltage (read-out with digital output); supports high frame rates, low overall electronics noise
Both technologies possess inherent advantages, so the question arises about what unique performance characteristics arise from each choice. CCDs can have 100% fill factor that captures all incoming light, whereas part of the CMOS sensor is occupied by transistors and metal wiring associated with each pixel. Historically, CCDs provided higher quality images with low noise, at affordable prices. Recent design advancements and processing techniques now advance CMOS sensor performance so it is a viable choice for some applications. Note that CCDs still maintain an advantage for binning in terms of signal-to-noise. However, CMOS chips can scale the number of read-out ports and achieve very high frame rates.
Step 4) Transfer signal and read-out image
When a charge converts to voltage, you typically generate noise
- CCD: Transfer data out of serial register
- CMOS: Converts to voltage per pixel
It is very important to optimize read-out noise (higher voltages) and speed (multi-port and fast read times) for CCDs.
- Optimize controller for low read-noise; leverage multi-port read-outs for faster speed
- Interline CCDs with binning have the fastest readouts (fps) – up to 30 fps due to 100% duty cycle
CMOS typically is seen as a fast sensor because you can run in rolling shutter mode verses the slow global shutter mode.
The cutting-edge counting camera for groundbreaking imaging, diffraction, and in-situ studies.
The only fully integrated hybrid-pixel electron detector with the Gatan Microscopy Suite software for advanced electron diffraction studies.
Gatan’s latest CMOS camera that with its resolution, speed, and ease of use will revolutionize electron microscopy.
Facilitate your HREM assays by automatically adjusting the critical imaging parameters of a TEM microscope focus, stigmation, and beam tilt.
DigitalMicrograph, also known as Gatan Microscopy Suite, drives your digital cameras and surrounding components to support key applications including tomography, in-situ, spectrum and diffraction imaging, plus more.
Delivers the efficiency and high-throughput data collection that you expect from Latitude software to MicroED studies.
Digital beam control and image processing to enhance the photographic quality of your digital images.
Diffraction analysis package (DIFPACK) to automate the selection area of your electron diffraction (SAED) patterns and high resolution lattice images of crystalline samples.
A powerful tool that adds 4D STEM diffraction capabilities to your existing Gatan in-situ camera.
Sets a new standard for the efficient, high-throughput collection of low-dose, single-particle, cryo-EM datasets from Gatan’s cameras.
The high-performance scintillator camera that elevates your everyday transmission electron microscopy
- Orientation map generated with STEMx OIM
- GIF Continuum K3 IS: Advanced Direct Detection for In-Situ Chemical Analysis
- Capturing, Processing, and Synchronizing In-Situ EELS Data
- Continuum IS: Versatile time-resolved data collection webinar
- Continuously acquired 4D STEM and EELS spectrum images for in-situ microscopy webinar
- 4D STEM and Virtual Aperture Imaging with ClearView
- Image samples at the desired orientation with TruAlign
- Better resolve faint, high-resolution diffraction spots with ClearView Frame Control mode
- High signal-to-noise TEM imaging with ClearView Frame Control mode
- Live drift correction during imaging acquisition
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Yan, L.; Zhang, Y.; Ge, J.; Zheng, L.; Gao, Y.; Wang, T.; Jia, Z.; Wang, H.; Huang, Y.; Li, M.; Wang, Q.; Ra, Z.; Lou, Z.
Du, S.; Cao, Y.; Zhu, Q.; Yu, P.; Qi, F.; Wang, G.; Du, X.; Bao, L.; Deng, W.; Zhu, H.; Liu, J.; Nie, J.; Zheng, Y.; Liang, H.; Liu, R.; Gong, S.; Xu, H.; Yisimayi, A.; Qin, C.
Piccoli, L.; Park, Y. -J.; Tortorici, M. A.; Czudnochowski, N.; Walls, A. C.; Beltramello, M.; Silacci-Fregni, C.; Pinto, D.; Rosen, L. E.; Bowen, J. E.; Acton, O. J.; Jaconi, S.; Guarino, B.; Minola, A.; Zatta, F.; Sprugasci, N.; Bassi, J.; Peter, A.; De Marco, A.; Nix, J. C.; Mele, F.; Jovic, S.; Rodriguez, B. F.; Gupta, S. V.; Jin, F.; Piumatti, G.; Presti, G. L.; Pellanda, A. F.; Biggiogero, M.; Tarkowski, M.; Pizzuto, M. S.; Cameroni, E.; Havenar-Daughton, C.; Smithey, M.; Hong, D.; Lepori, V.; Albanese, E.; Ceschi, A.; Bernasconi, E.; Elzi, L.; Ferrari, P.; Garzoni, C.; Riva, A.; Snell, G.; Sallusto, F.; Fink, K.; Virgin, H. W.; Lanzavecchia, A.; Corti, D.; Veesler, D.
Guo, L.; Bi, W.; Wang, X.; Xu, W.; Yan, R.; Zhang, Y.; Zhao, K.; Li, Y.; Zhang, M.; Cai, X.; Jiang, S.; Xie, Y.; Zhou, Q.; Lu, L.; Dang, B.
Cathode-electrolyte interphase in lithium batteries revealed by cryogenic electron microscopy
Zhang, Z.; Yang, J.; Huang, W.; Wang, H.; Zhou, W.; Li, Y.; Li, Y.; Xu, J.; Huang, W.; Chiu, W.
Free fatty acid binding pocket in the locked structure of SARS-CoV-2 spike protein
Toelzer, C.; Gupta, K.; Yadav, S. K. N.: Borucu, U.; Davidson, A. D.; Williamson, M. K.; Shoemark, D. K.; Garzoni, F.; Staufer, O.; Milligan, R.; Capin, J.; Mulholland, A. J.; Spatz, J.; Fitzgerald, D.; Berger, I.; Schaffitzel, C.
An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive spike
Schoof, M.; Faust, B.; Saunders, R. A.; Sangwan, S.; Rezelj, V.; Hoppe, N.; Boone, M.; Billesbølle, C. B.; Puchades, C.; Azumaya, C. M.; Kratochvil, H. T.; Zimanyi, M.; Deshpande, I.; Liang, J.; Dickinson, S.; Nguyen, H. C.; Chio, C. M.; Merz, G. E.; Thompson, M. C.; Diwanji, D.; Schaefer, K.; Anand, A. A.; Dobzinski, N.; Zha, B. S.; Simoneau, C. R.; Leon, K.; White, K. M.; Chio, U. S.; Gupta, M.; Jin, M.; Li, F.; Liu, Y.; Zhang, K.; Bulkley, D.; Sun, M.; Smith, A. M.; Rizo, A. N.; Moss, F.; Brilot, A. F.; Pourmal, S.; Trenker, R.; Pospiech, T.; Gupta, S.; Barsi-Rhyne, B.; Belyy, V.; Barile-Hill, A. W.; Nock, S.; Liu, Y.; Krogan, N. J.; Ralston, C. Y.; Swaney, D. L.; García-Sastre, A.; Ott, M.; Vignuzzi, M.; QCRG Structural Biology Consortium; Walter, P.; Manglik, A.
Custódio, T. F.; Das, H.; Sheward, D. J.; Hanke, L.; Pazicky, S.; Pieprzyk, J.; Sorgenfrei, M.; Schroer, M. A.; Gruzinov, A. Y.; Jeffries, C. M.; Graewert, M. A.; Svergun, D. I.; Dobrev, N.; Remans, K.; Seeger, M. A.; McInerney, G. M.; Murrell, B.; Hällberg, B. M.; Löw, C.
The architecture of inactivated SARS-CoV-2 with postfusion spikes revealed by cryo-EM and cryo-ET
Liu, C.; Mendonça, L.; Yang, Y.; Gao, Y.; Shen, C.; Liu, J.; Ni, T.; Ju, B.; Liu, C.; Tang, X.; Wei, J.; Ma, X.; Zhu, Y.; Liu, W.; Xu, S.; Liu, Y.; Yuan, J.; Wu, J.; Liu, Z.; Zhang, Z.; Liu, L.; Wang, P.; Zhang, P.
Zhou, T.; Teng, I. -T.; Olia, A. S.; Cerutti, G.; Gorman, J.; Nazzari, A.; Shi, W.; Tsybovsky, Y.; Wang, L.; Wang, S.; Zhang, B.; Zhang, Y.; Katsamba, P. S.; Petrova, Y.; Banach, B. B.; Fahad, A. S.; Liu, L.; Lopez Acevedo, S. N.; Madan, B.; de Souza, M. O.; Pan, X.; Wang, P.; Wolfe, J. R.; Yin, M.; Ho, D. D.; Phung, E.; DiPiazza, A.; Chang, L. A.; Abiona, O. M.; Corbett, K. S.; DeKosky, B. J.; Graham, B. S.; Mascola, J. R.; Misasi, J.; Ruckwardt, T.; Sullivan, N. J.; Shapiro, L.; Kwong, P. D.
Kratish, Y.; Nakamuro, T.; Liu, Y.; Li, J.; Tomotsuka, I.; Harano, K.; Nakamura, E.; Marks, T. J.
InsteaDMatic: Towards cross-platform automated continuous rotation electron diffraction
Roslova, M.; Smeets, S.; Wang, B.; Thersleff, T.; Xu, H.; Zou, X.
In situ TEM study of crystallization and chemical changes in an oxidized uncapped Ge2Sb2Te5 film
Singh, M. K.; Ghosh, C.; Miller, B.; Kotula, P. G.; Tripathi, S.; Watt, J.; Bakan, G.; Silva, H.; Carter, C. B.
Inside polyMOFs: Layered structures in polymer-based metal–organic frameworks
Bentz, K. C., Gnanasekaran, K., Bailey, J. B. Ayala, S., Tezcan, F. A., Gianneschi, N. C., Cohen, S. M.
Aryl radical addition to curvatures of carbon nanohorns for single-molecule level molecular imaging
Kamei, K.; Shimizu, T.; Harano, K.; Nakamura, E.
Kim, Y. -J.; Lee, Y.; Kim, K.; Kwon, O. -H.
Effect of adventitious carbon on pit formation of monolayer MoS2
Park, S.; Siahrostami, S.; Park, J.; Mostaghimi, A. H. B.; Kim, T. R.; Vallez, L.; Gill, T. M.; Park, W.; Goodson, K. E.; Sinclair, R.; Zheng, X.
Stuckner, J.; Shimizu, T.; Harano, K.; Nakamura, E.; Murayama, M.
Gnanasekaran, K., Vailonis, K. M., Jenkins, D. M., Gianneschi, N. C.
Langer, L. M.; Gat, Y.; Bonneau, F.; Conti, E.
Shimizu, T.; Lungerich, D.; Stuckner, J.; Murayama, M.; Harano, K.; Nakamura, E.
Decoding the stoichiometric composition and organisation of bacterial metabolosomes
Yang, M.; Simpson, D. M.; Wenner, N.; Brownridge, P.; Harman, V. M.; Hinton, J. C. D.; Beynon, R. J.; Liu , L. -N.
1D to 2D transition in tellurium observed by 4D electron microscopy
Londoño-Calderon, A.; Williams, D. J.; Ophus, C.; Pettes, M. T.
Murthy, A. A.; Stanev, T. K.; dos Reis, R.; Hao, S.; Wolverton, C.; Stern, N. P.; Dravid, V. P.
Unveiling the microscopic origins of phase transformations: An in situ TEM perspective
Yu, L.; Hudak, B. M.; Ullah, A.; Thomas, M. P.; Porter, C. C.; Thisera, A.; Pham, R. H.; Goonatilleke, M. D. A.; Guiton, B. S.
Ogata, A. F.; Rakowski, A. M.; Carpenter, B. P.; Fishman, D. A.; Merham, J. G.; Hurst, P. J.; Patterson, J. P.
Thickness and defocus dependence of inter-atomic electric fields measured by scanning diffraction
Addiego, C.; Gao, W.; Pan, X.
Errokh, A.; Magnin, A.; Putaux, J. -L.; Boufi, S.
Nam, K. W.; Park, S. S.; dos Reis, R.; Dravid, V. P. Kim, H.; Mirkin, C. A.; Stoddart, J. F.
Gao, W.; Addiego, C.; Wang, H,; Yan, X.; Hou, Y.; Ji, D.; Heikes, C.; Zhang, Y.; Li, L.; Huyan, H.; Blum, T.; Aoki, T.; Nie, Y.; Schlom, D.; Wu, R.; Pan, X.
Buffin, S.; Peubez, I.; Barrière, F.; Nicolaï, M., -C.; Tapia, T.; Dhir, V.; Forma, E.; Sève, N.; Legastelois, I.
Recent progress of in situ transmission electron microscopy for energy materials
Chao Zhang, C.; Firestein, K. L.; Fernando, J. F. S.; Siriwardena, D.; von Treifeldt, J. E.; Golberg, D.
Electron microdiffraction reveals the nanoscale twist geometry of cellulose nanocrystals
Ogawa, Y.
Atomistic structures and dynamics of prenucleation clusters in MOF-2 and MOF-5 syntheses
Xing, J.; Schweighauser, L.; Okada, S.; Harano, K.; Nakamura, E.
Quantifying inactive lithium in lithium metal batteries
Fang, C.; Li, J.; Zhang, M.; Zhang, Y.; Yang, F.; Lee, J. Z.; Lee, L. M. -H.; Alvarado, J.; Schroeder, M. A.; Yang, Y.; Lu, B.; Williams, N.; Ceja, M.; Yang, L.; Cai, M.; Gu, J.; Xu, K.; Wang, X.; Meng, Y. S.
Improved applicability and robustness of fast cryo-electron tomography data acquisition
Eisenstein, F.; Danev, R.; Pilhofer, M.
Solving a new R2lox protein structure by microcrystal electron diffraction
Xu, H.; Lebrette, H.; Clabbers, M. T. B.; Zhao, J.; Griese, J. J.; Zou, X.; Hogbom, M.
Li, X.; Wang, J.; Liu, X.; Liu, L.; Cha, D.; Zheng, X.; Yousef, A. A.; Song, K.; Zhu, Y.; Zhang, D.; Han, Y.
Formation of two-dimensional transition metal oxide nanosheets with nanoparticles as intermediates
Yang, J.; Zeng, Z.; Kang, J.; Betzler, S.; Czarnik, C.; Zhang, X.; Ophus, C.; Yu, C.; Bustillo, K.; Pan, M.; Qiu, J.; Wang, L. -W.; Zheng, H.
Wang, H.; Liu, Z.; Liu, H.; Guan, L.; Cao, X.; Zhang, Z.; Huang, Y.; Jin, C.
Mechanism of stress induced crystallization of polyethylene
Yakovlev, S.; Fiscus, D.; Brant, P.; Butler, J.; Bucknall, D. G.; Downing, K. H.
Cryo-EM structures of atomic surfaces and host-guest chemistry in metal-organic frameworks
Li, Y.; Wang, K.; Zhou, W.; Sinclair, R.; Chiu, W.; Cui, Y.
Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
Fan, X.; Wang, J.; Zhang, X.; Yang, Z.; Zhang, J. -C.; Zhao, L.; Peng, H. -L.; Lei, J.; Wang, H. -W.
Su, T.; Hood, Z. D.; Naguib, M.; Bai, L.; Luo, S.; Rouleau, C. M.; Ivanov, I. N.; Ji, H.; Qin, Z.; Wu, Z.
Thermal stability and irradiation response of nanocrystalline CoCrCuFeNi high-entropy alloy
Zhang, Y.; Tunes, M. A.; Crespillo, M. L.; Zhang, F.; Boldman, W. L.; Rack, P. D.; Jiang, L.; Xu, C.; Greaves, G.; Donnelly, S. E.; Wang, L.; Weber, W. J.
Tunes, M. A.; Harrison, R. W.; Donnelly, S. E.; Edmondson, P. D.
Factors affecting electron beam damage in calcite nanoparticles
Hooley, R.; Brown, A.; Brydson, R.
Low dose scanning transmission electron microscopy of organic crystals by scanning moiré fringes
S’ari, M.; Cattle, J.; Hondow, N.; Brydson, R.; Brown, A.
A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
Liu, Y.; Huynh, D. T.; Yeates, T. O.
In situ structures of polar and lateral flagella revealed by cryo-electron tomography
Zhu, S.; Schniederberend, M.; Zhitnitsky, D.; Jain, R.; Galán, J. E.; Kazmierczak, B. I.; Liu, J.
Le Ferrand, H.; Duchamp, M.; Gabryelczyk, B.; Cai, H.; Miserez, A.
Carbon polyaniline capacitive deionization electrodes with stable cycle life
Evans, S. F.; Ivancevic, M. R.; Wilson, D. J.; Hood, Z. D.; Adhikari, S. P.; Naskar, A. K.; Tsouris, C.; Paranthaman, M. P.
Off-axis electron holography for imaging the magnetic behavior of vortex-state minerals
Almeida, T. P.; Muxworthy, A. R.; Williams, W.; Kasama, T.; Kovács, A.; Dunin-Borkowski, R. E.
Connolly, M.; Zhang, Y.; Mahri, S.; Brown, D. M.; Ortuño, N.; Jordá-Beneyto, M.; Maciaszek, K.; Stone, V.; Fernandes, T. F.; Johnston, H. J.
Mechanical response of gasoline soot nanoparticles under compression: An in situ TEM study
Jenei, I. Z.; Dassenoy, F.; Epicier, T.; Khajeh, A.; Martinic, A.; Uy, D.; Ghaednia, H.; Gangopadhyay, A.
2D/2D heterojunction of Ti3C2/g-C3N4 nanosheets for enhanced photocatalytic hydrogen evolution
Su, T.; Hood, Z. D.; Naguib, M.; Bai, L.; Luo, S.; Rouleau, C. M.; Ivanov, I. N.; Ji, H.; Qin, Z.; Wu, Z.
Rodriguez-Caro, H.; Dragovic, R.; Shen, M.; Dombi, E.; Mounce, G.; Field, K.; Meadows, J.; Turner, K.; Lunn, D.; Child, T.; Southcombe, J. H.; Granne, I.
Nanoscale mosaicity revealed in peptide microcrystals by scanning electron nanodiffraction
Gallagher-Jones, M.; Ophus, C.; Bustillo, K. C.; Boyer, D. R.; Panova, O.; Glynn, C.; Zee, C., -T.; Ciston, J.; Mancia, K. C.; Minor, A. M.; Rodriguez, J. A.
Unconventional magnetization textures and domain-wall pinning in Sm--Co magnets
Pierobon, L.; Kovács, A.; Schäublin, R. E.; Wyss, U.; Dunin-Borkowski, R. E.; Löffler, J. F.; Charilaou, M.
Structural analysis of single nanoparticles in liquid by low-dose STEM nanodiffraction
Khelfa, A.; Byun, C.; Nelayah, J.; Wang, G.; Ricolleau, C.; Alloyeau, D.
Surface crystallization of liquid Au–Si and its impact on catalysis
Panciera, F.; Tersoff, J.; Gamalski, A. D.; Reuter, M. C.; Zakharov, D.; Stach, E. A.; Hofmann, S.; Ross, F. M
Layered-structure SbPO4/reduced graphene oxide: An advanced anode material for sodium ion batteries
Pan, J.; Chen, S.; Fu, Q.; Sun, Y.; Zhang, Y.; Lin, N.; Gao, P.; Yang, J.; Qian, Y.
2D transition metal carbides (MXenes) for carbon capture
Persson, I.; Halim, J.; Lind, H.; Hansen, T. W.; Wagner, J. B.; Näslund, L. -A.; Darakchieva, V.; Palisaitis, J.; Rosen J.; Persson, P. O. A.
2D transition metal carbides (MXenes) for carbon capture
Persson, I.; Halim, J.; Lind, H.; Hansen, T. W.; Wagner, J. B.; Näslund, L. -A.; Darakchieva, V.; Palisaitis, J.; Rosen J.; Persson, P. O. A.
Gao, W.; Wu, J.; Yoon, A.; Lu, P.; Qi, L.; Wen, J.; Miller, D. J.; Mabon, J. C.; Wilson, W. L.; Yang, H.; Zuo, J. -M.
Atomic step flow on a nanofacet
Harmand, J. -C.; Patriarche, G.; Glas, F.; Panciera, F.; Florea, I.; Maurice, J. -L.; Travers, L.; Ollivier, Y.
A high-entropy alloy with hierarchical nanoprecipitates and ultrahigh strength
Fu, Z.; Jiang, L.; Wardini, J. L.; MacDonald, B. E.; Wen, H.; Xiong, W.; Zhang, D.; Zhou, Y.; Rupert, T. J.; Chen, W.; Lavernia, E. J.
Pan, J.; Chen, S.; Zhang, D.; Xu, X.; Sun, Y.; Tian, F.; Gao, P.; Yang, J.
Jung, H. J.; Kim, D.; Kim, S.; Park, J.; Dravid, V. P.; Shin, B.
In situ kinetic and thermodynamic growth control of Au–Pd core–shell nanoparticles
Tan, S. F.; Bisht, G.; Anand, U.; Bosman, M.; Yong, X. E.; Mirsaidov, U.
Yasin, F. S.; Harvey, T. R.; Chess, J. J.; Pierce, J. S.; Ophus, C.; Ercius, P.; McMorran, B. J.
Zeng, L.; Gammer, C.; Ozdol, B.; Nordqvist, T.; Nygård, J.; Krogstrup, P.; Minor, A. M.; Jäger, W.; Olsson, E.
Yu, J.; Yuan, W.; Yang, H.; Xu, Q.; Wang, Y.; Zhang, Z.
Refinement and analysis of the mature Zika virus cryo-EM structure at 3.1 Å resolution
Sevvana, M.; Long, F.; Miller, A. S.; Klose, T.; Buda, G.; Sun, L.; Kuhn, R. J.; Rossmann, M. G.
Fernandez, S.; Ostraat, M. L.; Lawrence III, J. A.; Zhang, K.
Local nanoscale strain mapping of a metallic glass during in situ testing
Gammer, C.; Ophus, C.; Pekin, T. C.; Eckert, J.; Minor, A. M.
Xu, H. Lebrette, H.; Yang, T.; Hovmoller, S.; Hogbom, M.; Zou, X.
Hepp, S. E.; Borgo, G. M.; Ticau, S.; Ohkawa, T.; Welch, M. D.
Nanoparticle interactions guided by shape‐dependent hydrophobic forces
Tan, S. F.; Raj, S.; Bisht, G.; Annadata, H. V.; Nijhuis, C. A.; Král, P.; Mirsaidov, U.
Lutz, L.; Dachraoui, W.; Demortière, A.; Johnson, L. R.; Bruce∥, P. G.; Grimaud, A.; Tarascon, J. -M.
Destefani, T. A.; Onaga, G. L.; de Farias, M. A.; Percebom, A. M.; Sabadini, E.
Andrew, R. S.; Austin, B. F.; George, C. R.; Ronald, C. B.; James, M. J.
Solvent-free and biocompatible multiphased organic–inorganic hybrid nanocomposites
da Silva, L. C. E.; Germiniani, L. G. L.; Plivelic, T. S.; Gonçalves, M. C.
S.E.Gilliland III, Tengco, J. M. M.; Yang, Y.; Regalbuto, J. R.; Castano, C. E.; Gupton, B. F.
In situ nanobeam electron diffraction strain mapping of planar slip in stainless steel
Pekin, T. C.; Gammer, C.; Ciston, J.; Ophus, C.; Minor, A. M.
Direct microscopic analysis of individual C60 dimerization events: Kinetics and mechanisms
Okada, S.; Kowashi, S.; Schweighauser, L.; Yamanouchi, K.; Harano, K.; Nakamura, E.
Atomic structure of sensitive battery materials and interfaces revealed by cryo–electron microscopy
Li, Y.; Li.; Y.; Pei, A.; Yan, K.; Sun, Y.; Wu, C. -L; Joubert, L. -M.; Chin, R.; Koh, A. L.; Yu, Y.; Perrino, J.; Butz, B.; Chu, S.; Cui, Y.
Vertical graphene growth on SiO microparticles for stable lithium ion battery anodes
Shi, L.; Pang, C.; Chen, S.; Wang, M.; Wang, K.; Tan, Z.; Gao, P.; Ren, J.; Huang. Y.; Peng, H.; Liu, Z.
Ophus, C.; Ercius, P.; Huijben, M.; Ciston, J.
Distinct nanoscale reaction pathways in a sulfide material for sodium and lithium batteries
Boebinger, M. G.; Xu, M.; Ma, X.; Chen, H.; Unocic, R. R.; McDowell, M. T.
Koh, A. L.; Gidcumb, E.; Zhou, O.; Sinclair, R.
Local and transient nanoscale strain mapping during in situ deformation
Gammer, C.; Kacher, J.; Czarnik, C.; Warren, O. L.; Ciston, J.; Minor, A. M.
Practical aspects of diffractive imaging using an atomic-scale coherent electron probe
Chen, Z.; Weyland, M.; Ercius, P.; Ciston, J.; Zheng, C.; Fuhrer, M. S.; D'Alfonso. A. J.; Allen, L. J.; Findlay, S. D.
Highly mobile ferroelastic domain walls in compositionally graded ferroelectric thin films
Agar, J. C.; Damodaran, A. R.; Okatan, M. B.; Kacher, J.; Gammer, C.; Vasudevan, R. K.; Pandya, S.; Dedon, L. R.; Mangalam, R. V. K.; Velarde, G. A.; Jesse, S.; Balke, N.; Minor, A. M.; Kalinin, S. V.; Martin, L. W.
Diffraction contrast imaging using virtual apertures
Gammer, C.; Ozdol, V. B.; Liebscher, C. H.; Minor, A. M.
Strain mapping at nanometer resolution using advanced nano-beam electron diffraction
Ozdol, V. B.; Gammer,C.; Jin, X. G.; Ercius, P.; Ophus, C.; Ciston,J.; Minor, A. M.
Lear, P. V.; González-Touceda, D.; Couto, B. P.; Viaño, P.; Guymer, V.; Remzova, E.; Tunn, R.; Chalasani, A.; García-Caballero, T.; Hargreaves, I. P.; Tynan, P. W.; Christian, H. C.; Nogueiras, R.; Parrington, J.; Diéguez, C.
Nyquist frequency
Dose fractionation and motion correction
Improving DQE with counting and super-resolution