Frozen hydrated lipid vesicles
Image courtesy of Jessica Goodwin and Htet Khant, National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX
Methods
prepared using Cryoplunge™ 3 instrument
image was recorded using UltraScan 4000 camera
TEM magnification of 25 kx at 200 keV
electron dose of ~20 e-/Å2
910 multi-specimen single tilt cryo transfer holder
sample prepared on Quantifoil specimen support
used Solarus advanced plasma cleaner prior to freezing
