Frozen-hydrated image of the Ndc80 complex decorated microtubules
Image courtesy of Dr. Elizabeth Wilson‐Kubalek, Cell Biology, The Scripps Research Institute, La Jolla, CA
Methods
magnification 30kx
electron dose 40 e‐/Å2
specimens prepared on CFlat™ Protochips, Inc. CF‐2/1‐4C‐50 holey carbon grids
plasma cleaned using the Solarus® advanced plasma cleaning system
frozen hydrated preparations produced using Cryoplunge™ 3 instrument
image recorded at 100 kV on a TEM equipped with a model 626 70° single tilt liquid nitrogen cryo-transfer holder
